Development of a sensitive monoclonal antibody-based immunochromatographic strip for neomycin detection in milk

To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, conjugated to carrier protein prepare mAbs. We obtained six mAbs: mAb 1C6, 1D3, 2D3, 4D5, 5D1, 5H1. characterised mAbs by indirect competitive enzyme-linked immunosorbent assay selected most half-maximal inhibitory concentration (IC50). 4D5 (IC50 = 0.15 ng/mL) for development LFA. MAb was labelled with ACNPs (ACNPs-mAb 4D5) electrostatic absorption. Under optimised conditions, 5.4 ?g coupling 1 mL ACNPs, NEO-OVA at 80 ?g/mL, 3 ?L ACNPs-mAb were used develop The cut-off value 8 ng/mL. Therefore, our ACNPs-LFA is suitable on-site residues.